A suspension cell culture flask belongs to the technical field of biological cell culture.

Cell culture techniques refer to techniques for maintaining the growth of cells under in vitro conditions. According to the characteristics of whether the cells are attached to the support for growth, they are divided into attached type and suspension type. In culture, two methods of static culture and dynamic culture are mainly used. Under the condition of static culture, in order to ensure that the cells can have a sufficient gas environment, there is a requirement for the volume of the medium in the Cell Culture Flask. Under normal conditions, the ratio of the medium volume to the bottom area of the Cell Culture Flask is 0.2ml/cm2 ~0.5ml/cm2. After this ratio is exceeded, it is necessary to spin the flask or stir the culture flask for dynamic culture or subsurface aeration to meet the needs of the cells for gas.
The existing suspension culture flasks is composed of a bottle body and a bottle cap. The top of the suspension culture flask body is opened, and the bottle cap is screwed tightly, and the flask cap is usually breathable. However, in the case where only the bottle cap has gas permeability, the area for exchange with air is small, which limits the volume of medium added to the suspension culture flasks during cell culture, so that the suspension culture flasks needs to be replaced frequently during the culture process, increasing the Risk of cellular contamination.